Composite

Part:BBa_K5061161:Design

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


XylS K38R L224Q expression cassette in EP backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2724
    Illegal NgoMIV site found at 819
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

DNA synthesis, PCR, restriction digest and assembly

References